MAMMALIAN PROTEIN EXPRESSION
- codon optimization for mammalian cell expression and gene synthesis of DNA insert
- mutagenesis of an existing construct
- generation of mammalian protein expression construct(s) in a selected or client-specified vector
BACULOVIRUS-INDUCED INSECT PROTEIN EXPRESSION
- codon optimization for insect cell expression and gene synthesis of DNA insert
- mutagenesis of an existing construct
- subcloning of a DNA insert into a selected or client-specified baculovirus expression
Bacterial PROTEIN EXPRESSION
- codon optimization for bacterial expression and gene synthesis of DNA insert
- generation of bacterial expression construct(s) in a selected or client-specified vector
- transformation into an appropriate host and preparation
Fusion-protein Services
NanoExpo has extensive experience with making DNA constructs for fusion protein production, expressing and purifying custom fusion proteins in mammalian, insect and bacterial cells. With an optimized DNA construction system
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point. We offer mono, bi and tricistronic expression
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Generation of recombinant viruses
Recombinant baculovirus is produced following co-transfection of insect cells with a transfer plasmid containing the gene of interest and flashBAC™ baculovirus DNA. The recombinant virus harvested from the co-transfection is
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.