Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Generation of recombinant viruses
Recombinant baculovirus is produced following co-transfection of insect cells with a transfer plasmid containing the gene of interest and flashBAC™ baculovirus DNA. The recombinant virus harvested from the co-transfection is
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.