Fusion-protein Services

NanoExpo has extensive experience with making DNA constructs for fusion protein production, expressing and purifying custom fusion proteins in mammalian, insect and bacterial cells. With an optimized DNA construction system and fully optimized secretion signal and linker sequences, NanoExpo can efficiently complete a project from DNA construction and transient production of fusion proteins in selected expression system, to fusion protein purification. Standard fusion proteins include C- and N- terminal fusions with tags for easy and convenient purifcation by affinity chromatography in purification such as staphylococcus protein A, glutathione-S-transferase (gst), maltose-binding protein (mbp) and cellulose-binding protein. The recombinant fusion proteins are used most often as fusion partners for reporter constructs are β-galactosidase, luciferase, and fluorescent proteins such as green fluorescent protein (GFP), orange fluorescent protein (OFP) and yellow fluorescent protein (YFP). Fluorescent proteins such as green fluorescent protein (GFP) for direct observation of dynamic intracellular processes. Moreover Fusion proteins include inner insertions fusions for epitope presentation. NanoExpo has experience in designing artificial fusion proteins that can serve as antigen presenting platforms, catcher platforms, delivery platforms.