- codon optimization for bacterial expression and gene synthesis of DNA insert
- generation of bacterial expression construct(s) in a selected or client-specified vector
- transformation into an appropriate host and preparation of glycerol stocks
- screening studies for the best-expressing protein variant
- optimization of growth conditions (host, induction, media, temperature, additives) to drive either soluble or inclusion bodies expression
- soluble vs. inclusion bodies expression assessment by SDS-PAGE/Coomassie or Western blot
- bacterial paste scale up production
- inclusion bodies wash and recovery
- recombinant protein purification from soluble lysate fraction
- recombinant protein purification from inclusion bodies by refolding
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