Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The recombinant protein can be purified and characterized or shipped directly as a crude product.
Optimisation of expression
To maximize protein production yield, the optimal insect cell type, promotor type, temperature, M.O.I. and harvest time will be determined.
Titration of baculoviruses
NanoExpo has developed a quick and easy system for the accurate titration of baculovirus stocks based on probe-based quantitative PCR (qPCR). In this approach, a freshly amplified baculovirus inoculum is
Generation of recombinant viruses
Following transfection of insect cells with a transfer plasmid containing the gene of interest and baculovirus DNA, recombinant baculovirus is generated. The recombinant virus is then used to produce an
Gene synthesis and cloning
We can coordinate the design and synthesis of any gene and use any plasmid that you provide as a starting point. Additionally, we offer our original NanoMax plasmids that