- codon optimization for insect cell expression and gene synthesis of DNA insert
- mutagenesis of an existing construct
- subcloning of a DNA insert into a selected or client-specified baculovirus expression vector
- virus generation, amplification and cloning by limited dilution or plaque purification
- generation of high-titer virus stocks
- SDS-PAGE, Western, ELISA or functional expression analysis of cell lysates and conditioned media for intracellular and secreted protein products respectively
- secreted or intracellular expression in Sf9, ExpiSF, Sf21 and HiFive insect cells
- expression optimization studies: MOI, time course, growth media
- large-scale insect cell culture for conditioned media or cell pellet production
- recombinant protein purification from conditioned media or cell pellet
We are specialists in biotechnology, bioengineering, molecular biology , cell culture and virology.