MAMMALIAN PROTEIN EXPRESSION

Protein production, MAMMALIAN PROTEIN EXPRESSION, Virology
  • codon optimization for mammalian cell expression and gene synthesis of DNA insert
  • mutagenesis of an existing construct
  • generation of mammalian protein expression construct(s) in a selected or client-specified vector
  • endotoxin-free plasmid DNA preparation for transient or stable transfections
  • small-scale trial to assess recombinant protein expression and/or to optimize transient transfection conditions
  • various transient transfection reagent options
  • generation of stably-transfected cell pool
  • expression analysis by SDS-PAGE, Western, ELISA or functional assay
  • secreted or intracellular expression in CHO, HEK293 or client-specified cells
  • adherent or suspension cell growth options
  • large-scale mammalian cell culture for conditioned media or cell pellet production
  • recombinant protein purification from conditioned media or cell pellet