The process of protein purification is crucial to the study of proteins. The purity and quality of the isolated protein of interest are crucial factors in the research of protein structure, function, and interactions. Protein extraction buffer enables
The Protein extraction buffer enables efficient, small-scale, total soluble protein extraction from mammalian cells and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. Integral membrane proteins having one or two membrane-spanning domains are effectively solubilized and isolated from cytosolic proteins.
These formulation, which has been proven effective in numerous tissue types and cell lines, typically does not require mechanical cell rupture. These extracts can be used for many downstream applications, such as protein assays, immunoprecipitation, protein purification, immunoassays, western blotting, ELISA, enzyme assays, BCA assays and immunoprecipitation.