Cell Lysis Buffer (10x) is a high-quality, ready-to-use cell lysis solution for total protein extraction from cultured eukaryotic (mammalian and insect) cells. The buffer uses detergent-based lysis, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating proteins from cell cultures. It efficiently lyses cultured cells, such as adherent cultures and suspension cells in pellet form. The popular chemical aids in the extraction of membrane, nuclear, and cytoplasmic proteins and is useful for a variety of applications such as ELISA, western blotting, protein purification, BCA Protein Assay, and immunoassays. This cell lysis buffer formulation is compatible with any protease inhibitor cocktail that must be added before usage to avoid proteolysis and maintain protein phosphorylation.