Generation of recombinant viruses
Following transfection of insect cells with a transfer plasmid containing the gene of interest and baculovirus DNA, recombinant baculovirus is generated. The recombinant virus is then used to produce an
Gene synthesis and cloning
We can coordinate the design and synthesis of any gene and use any plasmid that you provide as a starting point. Additionally, we offer our original NanoMax plasmids that provide
VLPs visualization
TEM services
Visualisation of VLPs is the crucial part of the technology of production. NanoExpo offer visualisation of VLPs via treansmision electron microscopy. We use transmission electron microscope Tecnai G2 Spirit
VLPs purification
NanoExpo offer purification of VLPs in 2 steps or one step. Affinity chromatography can be followed by Size exclusion chromatography. One step purification include the pre-concentration and SEC. After purifitation
Test & Optimisation Of Expression
A quick test of expression undertaken at 48 hours of expression will provide a reasonable indication of whether a protein is expressed to reasonable levels or not, however, by varying
MAMMALIAN PROTEIN EXPRESSION
- codon optimization for mammalian cell expression and gene synthesis of DNA insert
- mutagenesis of an existing construct
- generation of mammalian protein expression construct(s) in a selected or client-specified vector
BACULOVIRUS-INDUCED INSECT PROTEIN EXPRESSION
- codon optimization for insect cell expression and gene synthesis of DNA insert
- mutagenesis of an existing construct
- subcloning of a DNA insert into a selected or client-specified baculovirus expression
Bacterial PROTEIN EXPRESSION
- codon optimization for bacterial expression and gene synthesis of DNA insert
- generation of bacterial expression construct(s) in a selected or client-specified vector
- transformation into an appropriate host and preparation
Fusion-protein Services
NanoExpo has extensive experience with making DNA constructs for fusion protein production, expressing and purifying custom fusion proteins in mammalian, insect and bacterial cells. With an optimized DNA construction system
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point. We offer mono, bi and tricistronic expression
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The protein produced can be purified or shipped direct as crude product.
Optimisation of expression
To maximise yield, optimal multiplicity of infection (MOI) and time of harvest is determined for the protein.
Generation of recombinant viruses
Recombinant baculovirus is produced following co-transfection of insect cells with a transfer plasmid containing the gene of interest and flashBAC™ baculovirus DNA. The recombinant virus harvested from the co-transfection is
Gene synthesis and cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.
Analysis of interaction
NanoExpo is providing protein interaction services using PeptAIm – a unique, AI-powered, bioinformatics platform that supports the design and development of molecular diagnostic tests. PeptAIm enables the selection of customized
Antibody production
NanoExpo provides rabbit polyclonal antisera production services as well as antigen synthesis, antibody purification, and analysis. To meet the needs of each individual customer, projects can be tailored to client-specific
Expression of recombinant protein
Following the optimisation 1L cell cultures are harvested under the best conditions. The recombinant protein can be purified and characterized or shipped directly as a crude product.
Optimisation of expression
To maximize protein production yield, the optimal cell type, promotor type, temperature and harvest time will be determined.
Gene synthesis and cloning
We can coordinate the design and synthesis of any gene and use any plasmid that you provide as a starting point. additionally, we offer our original NanoMax plasmids that